152 0 obj /D [29 0 R /FitR 52 553 290 553] Simple heart-rate measurement in zebrafish can provide predictive information regarding interactions of chemicals with the components of the cardiac functional regulatory network 15. Zebrafish embryos were exposed to SV47 or DB14 from 6 to 120 h post fertilization … /D [28 0 R /FitR 52 513 290 513] endobj 59 0 obj Fluorescence techniques used to probe biomolecular dynamics and interactions of molecules in live zebrafish embryos are therefore highly sought … /D [19 0 R /FitR 52 514 290 514] /D [28 0 R /FitR 52 224 290 224] << Zebrafish or zebra danio (danio rerio) are seen as one of the latest \"models\" for vertebrate embryological development studies. endobj Zebrafish-Mycobacterium marinum model for mycobacterial pathogenesis. 108 0 obj 21138/ARC_/Arthritis Research UK/United Kingdom, MR/S00551X/1/MRC_/Medical Research Council/United Kingdom, 098411/Z/12/Z/WT_/Wellcome Trust/United Kingdom, 206444/Z/17/Z/WT_/Wellcome Trust/United Kingdom, Lam PY, Fischer RS, Shin WD, Waterman CM, Huttenlocher A. << >> endobj /Thumb 406 0 R 28 0 R] << >> Both dyes have shown potential for toxicity; however, there is no comprehensive understanding of their effects. endobj /D [28 0 R /FitR 307 602 545 602] >> endobj endobj endobj /D [29 0 R /FitR 307 672 545 672] << << FEMS Microbiol Lett. 18 0 obj PY - 2012. << /Annots [283 0 R 284 0 R 285 0 R 286 0 R 287 0 R 288 0 R 289 0 R 290 0 R 291 0 R 292 0 R /D [20 0 R /FitR 307 221 545 221] 120 0 obj /Limits [(CR67) (Section15)] << Its experimental virtues make it an ideal system with which to identify new bioactive molecules, and to assess their toxicity and teratogenicity at medium-to-high throughput. /TrimBox [0 -5.66927 595.276001 785.197021] One reason for the success of zebrafish as a model organism is its amenability to genetic manipulation. We outline broad applications of this method in: (i) visualizing the biomolecular distribution of whole embryos in three dimensions, (ii) resolving anatomical features at subcellular spatial resolution, (iii) biomolecular profiling and discrimination of wild type and ΔRD1 mutant Mycobacterium marinum strains in a zebrafish embryo model of tuberculosis and (iv) in vivo temporal monitoring of the wound response in living zebrafish embryos. /Dest (Section11) /Rotate 0 Low cost, easy handling, the requirement of small quantities of test compounds, and high t… >> 142 0 obj endobj >> Early zebrafish embryos are less than 1 mm in diameter, allowing several embryos to fit easily in a single well of a 384-well plate. /D [27 0 R /FitR 307 272 545 272] /D [27 0 R /FitR 307 322 545 322] << /Next 169 0 R << 158 0 obj >> Here, we investigated the applicability of pollutant injection into the yolk of early zebrafish (Danio rerio) … /Type /Page Fig. 2018 Nov 1;150:32-41. doi: 10.1016/j.ymeth.2018.07.004. (HypFig3) 139 0 R (HypFig4) 140 0 R (HypSec1) 141 0 R (HypSec2) 142 0 R (HypSec3) 143 0 R De Luca, E. et al. /Resources 376 0 R << << /Count -5 >> (CR71) 99 0 R (CR72) 100 0 R (CR73) 101 0 R (CR74) 102 0 R (CR75) 103 0 R /D [29 0 R /FitR 52 652 290 652] /D [29 0 R /FitR 52 702 290 702] << /TrimBox [0 -5.66927 595.276001 785.197021] endobj 2013;783:251-66. doi: 10.1007/978-1-4614-6111-1_13. INTRODUCTION. 85 0 obj /G12 175 0 R /Prev 418 0 R The SiO(2) particles were distributed throughout the outer surface … endobj Hence, this review intends to provide a comprehensive account of available scientific evidence of the application of the zebrafish embryos to assess the herbal toxicity. << 60 0 obj Nano-HA aggregated into the biggest particles around the membrane protein and then caused a little toxicity to development of zebrafish embryos. >> /Thumb 243 0 R /D [21 0 R /FitR 52 171 290 171] endobj endobj /D [29 0 R /FitR 307 224 545 224] << /D [20 0 R /FitR 0 786 596 0] /D [29 0 R /FitR 307 174 545 174] /Parent 10 0 R /Thumb 377 0 R /Resources 354 0 R Schematic overview of the confocal…, Fig. 83 0 obj /ExtGState << endobj endobj endobj << << << endobj Despite these caveats, several features of zebrafish—including the available genetic resources and transparency of the embryo—have proven useful in a variety of research applications. /Type /Page endobj 293 0 R 294 0 R 295 0 R 296 0 R 297 0 R 298 0 R 299 0 R 300 0 R 301 0 R 302 0 R 3 (A), the use of the AL treatment has led to an apparently significant lethality rate of the early zebrafish embryos, but fortunately with development progresses of embryos, the death rate decreased. 121 0 obj << /F (folder.joboptions) 2 0 obj /Names [(Section16) 158 0 R (Section17) 159 0 R (Section18) 160 0 R (Section19) 161 0 R (Section210) 162 0 R 6. cRSI of living zebrafish embryo wound response. endobj 137 0 obj /D [28 0 R /FitR 307 224 545 224] /ca .2 VEGF Inhibitor Treatment >> /Type /Page 95 0 obj Confocal Raman spectroscopic imaging analysis of zebrafish embryo model of tuberculosis. /D [20 0 R /FitR 307 221 545 221] endobj endobj /Next 415 0 R endobj 138 0 obj /CreationDate (D:20080715065123+08'00') >> << (Section12) 154 0 R (Section13) 155 0 R (Section14) 156 0 R (Section15) 157 0 R] << 3. << endobj 139 0 obj << /Names 3 0 R 141 0 obj endobj /D [20 0 R /FitR 307 246 545 246] /D [28 0 R /FitR 52 672 290 672] Zebrafish embryos were kept in a 28 °C incubator in E3 media (5 mM NaCl, 0.33 mM MgSO 4, 0.33 mM CaCl 2, 0.17 mM KCl, and 0.1% methylene blue). << /LW 1 /Limits [(Section16) (maintitle)] These embryos have the great advantage that they develop as \"see through\" embryos, that is, all internal development can be clearly observed from the outside in the living embryo. 23 0 obj the laboratory. 87 0 obj 150 0 obj /Thumb 325 0 R Zebrafish embryos were infected with P. aeruginosa to demonstrate that phage therapy is effective against P. aeruginosa infections as it reduces lethality, bacterial burden and pro-inflammatory immune response in CF embryos. Adult zebrafish (Danio rerio) were maintained according to standard laboratory procedures.19 Embryos and larvae were raised in embryo water (5 mM NaCl, 0.17 mM KCl, 0.33 mM CaCl 2, 0.33 mM MgSO 4) in an incubator at 28.5°C. uuid:4ba6ad0e-11ce-4e40-ae92-e950148537ca endobj 1- Collect embryos at the one-cell stage in a 35 mm Petri dish with E3 medium (100 embryos per condition at least). 11 0 obj 2014;1124:219–233. /Kids [18 0 R 19 0 R 20 0 R 21 0 R 22 0 R 23 0 R 24 0 R 25 0 R 26 0 R 27 0 R /Title Zebrafish eggs are fertilized and develop outside of the mother's body making visualization with high resolution imaging easier for researchers. The utility of fish embryos for pesticide hazard assessment was investigated by comparing published zebrafish embryo toxicity data from pesticides with median lethal concentration 50% (LC50) data for juveniles of 3 commonly tested fish species: rainbow trout, bluegill sunfish, and sheepshead minnow. The 18-mer ncPNAs were attached to a short complementary 2‘-OMe RNA sense strand (sRNA) via a heterobifunctional photocleavable linker (PL). >> The epigenetics of zebrafish embryos and different tissues in adult zebrafish are modulated in development [138, 149, 150] and in cancer [145, 151]. /D [28 0 R /FitR 307 293 545 293] High-resolution confocal Raman spectroscopic imaging…. /D [25 0 R /FitR 307 546 545 546] 37 0 obj /D [27 0 R /FitR 307 543 545 543] /D [27 0 R /FitR 307 492 545 492] endobj peutic applications [4,5]. endobj endobj /D [28 0 R /FitR 52 144 290 144] A short description of the preparation of zebrafish embryos for FCS measurements is included. >> The protocol detailed in this work describes the application of phage therapy against Pseudomonas aeruginosa infection in CF zebrafish embryos. 104 0 obj << endobj /Type /Catalog endobj NIH of embryos, ranging from insects to vertebrates (e.g. /D [28 0 R /FitR 52 423 290 423] /D [19 0 R /FitR 52 334 290 334] /F 414 0 R /D [27 0 R /FitR 307 642 545 642] 167 0 obj endobj 112 0 obj 3. /D [29 0 R /FitR 52 353 290 353] << << >> Overall, this study demonstrates the application of confocal Raman spectroscopic imaging for the comparative bimolecular analysis of fully intact and living zebrafish embryos. 125 0 obj N2 - As an emerging model for toxicological studies, zebrafish has been explored for nanotoxicity assessment. 156 0 obj 28 0 obj << 58 0 obj /D [27 0 R /FitR 52 142 290 142] �+Sl�V����˗���Gޗ"���%{O���ȇ�,Ej籬s�/�rF �}S��t���6�Z����;[�� /D [19 0 R /FitR 307 321 545 321] endobj /D [27 0 R /FitR 307 622 545 622] endobj To facilitate the application of … >> /D [23 0 R /FitR 52 508 290 508] << (CR59) 85 0 R (CR6) 86 0 R (CR60) 87 0 R (CR61) 88 0 R (CR62) 89 0 R /D [28 0 R /FitR 307 353 545 353] endobj << endobj /BM /Normal /CropBox [0 -5.66927 595.276001 785.197021] /D [24 0 R /FitR 0 786 596 0] /Resources 412 0 R /Parent 5 0 R << /D [29 0 R /FitR 52 383 290 383] >> /Prev 417 0 R 49 0 obj More recently, the zebrafish embryo has been applied … /Creator (3B2 Total Publishing System 8.07e/W Unicode ) >> >> << AU - Chen, Yongsheng. /Rotate 0 /Names [(CR1) 30 0 R (CR10) 31 0 R (CR100) 32 0 R (CR11) 33 0 R (CR12) 34 0 R /Contents 203 0 R /D [19 0 R /FitR 52 421 290 421] AB - As an emerging model for toxicological studies, zebrafish has been explored for nanotoxicity assessment. /Resources << >> /D [27 0 R /FitR 307 372 545 372] >> 388 0 R 389 0 R] >> endobj /LC 0 /Thumb 282 0 R /D [19 0 R /FitR 307 383 545 383] 44 0 obj << << endobj Would you like email updates of new search results? << 97 0 obj << (CR40) 65 0 R (CR41) 66 0 R (CR42) 67 0 R (CR43) 68 0 R (CR44) 69 0 R endobj 192 0 R 193 0 R 194 0 R 195 0 R 196 0 R 197 0 R 198 0 R 199 0 R 200 0 R 201 0 R /First 9 0 R >> 162 0 obj << 53 0 obj /Outlines 4 0 R /D [27 0 R /FitR 307 582 545 582] 2014-11-13T14:02:24+01:00 Zebrafish heg1 ∆25 embryos at 48 hpf were treated with each drug to determine the optimal concentration for recovery. endobj >> application/pdf << /Contents 323 0 R << /D [28 0 R /FitR 52 572 290 572] /First 16 0 R /D [29 0 R /FitR 52 124 290 124] << << endobj /D [27 0 R /FitR 307 692 545 692] /Metadata 2 0 R << /EmbeddedFiles 8 0 R 117 0 obj Spinning disk confocal imaging of neutrophil migration in Zebrafish. << 164 0 obj 73 0 obj (CR9) 119 0 R (CR90) 120 0 R (CR91) 121 0 R (CR92) 122 0 R (CR93) 123 0 R endobj /D [27 0 R /FitR 307 92 545 92] /Contents 241 0 R This method leads to uniform distribution of injected material throughout the embryo and can produce visible phenotypes resulting from the overexpression or loss of a protein. 20 0 obj /D [28 0 R /FitR 52 702 290 702] >> Biomolecular profiling and discrimination of…. Position of 10x images relative to 1 dpf zebrafish embryo. 2012;485:471–477. /Annots [393 0 R 394 0 R 395 0 R 396 0 R 397 0 R 398 0 R 399 0 R] This site needs JavaScript to work properly. /D [19 0 R /FitR 307 383 545 383] /CA 1 /Limits [(CR1) (CR66)] >> /SMask 419 0 R /EF << >> endobj /TrimBox [0 -5.66927 595.276001 785.197021] /Parent 5 0 R 66 0 obj However, factors such as the small cell size, high cell deformation … endobj << >> /MediaBox [0 -5.66927 595.276001 785.197021] endobj /MediaBox [0 -5.66927 595.276001 785.197021] The application of a chemical dispersant increased the acute toxicity most likely due to a higher bioavailability of dissolved and … 3B2 Total Publishing System 8.07e/W Unicode endobj 90 0 obj /CropBox [0 -5.66927 595.276001 785.197021] /Type /Page Zebrafish xenograft models of cancer and metastasis for drug discovery. /D [29 0 R /FitR 52 303 290 303] /D [27 0 R /FitR 52 219 290 219] endobj %���� 264 0 R 265 0 R 266 0 R 267 0 R 268 0 R 269 0 R 270 0 R 271 0 R 272 0 R 273 0 R /Resources 281 0 R correction of cell membrane movement during measurement and correction of spectral cross-talk of fluorescent proteins. >> /D [22 0 R /FitR 0 786 596 0] >> >> << /D [19 0 R /FitR 52 334 290 334] >> Y1 - 2012. endobj 1. /X8 176 0 R /D [29 0 R /FitR 307 533 545 533] endobj 39 0 obj >> 94 0 obj >> << Figure 2. << << endobj pearing in the development of zebrafish, Danio (Brachydanio) rerio, embryos fertilized simultaneously in vitro (C. Walker and G. Streisinger, in Westerfield, 1994) and incubated at an optimal temperature with- out crowding (28.S0C, 5-10 embryos/ml). 84 0 obj The optical transparency of zebrafish embryos and their amenability to genetic manipulation (e.g., gene knockdown or expression of fluorescent proteins in specific tissues/organs) are extremely advantageous to precisely track the release dynamics of loaded drugs and investigate the underlying mechanisms of nano–bio interactions, which can greatly facilitate the optimization of nanomedicine formulations and predict the efficacy of … >> << endobj /D [28 0 R /FitR 52 303 290 303] >> 98 0 obj /Dest (Section17) 16 0 obj /D [29 0 R /FitR 52 682 290 682] /Type /Pages doi: 10.1038/nature11057. >> 145 0 obj endobj Fig. 9 0 obj >> endobj >> << Evaluation of 14 organic solvents and carriers for screening applications in zebrafish embryos and larvae. >> 171 0 obj << (CR22) 45 0 R (CR23) 46 0 R (CR24) 47 0 R (CR25) 48 0 R (CR26) 49 0 R Position of 10x images relative to 1 dpf zebrafish embryo. 1. /D [20 0 R /FitR 52 271 290 271] >> endobj There is a pertinent need to renew scientific enthusiasm toward incorporating the zebrafish embryotoxicity model in toxicological evaluation on herbal medicine. 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